R74
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Description
Description: The R74 line was derived by co-cultivation of 18 day-old Lewis rat embryonic fibroblasts with cells of a chicken positive for Rous sarcoma virus-33 (RSV-33). This cell population is clearly distinguishable from RT1 and R9 as it is characterised by the presence of fibroblastoid cells that tend to round up and form clusters of refractile cells. R74 is also tumourigenic in chickens and rats.
Also Known As:
Species: Rat
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr M Grofova/Dr S Blahova, Cancer Research Institute, Bratislava
Originator: Yes
References: Folia Biol (Praha) 1992;38:90
Hyperlink to ECACC Cell Line Data Sheet: 93120215
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