Description: The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

Also Known As:

Species: Rat

Tissue: Choroid plexus

Growth Properties: Adherent

Morphology: Endothelial

Growth Medium: DMEM, 2mM Glutamine, 10% FCS, 0.1% Epidermal growth factor, 1% insulin/transferrin/selenium mixture

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Release Conditions: No

Depositor: Bruno Corman, Service de Biologie Cellulaire, CEA Saclay, Gif-sur-Yvette 91191, Cedex, France

Originator: Yes

References: Battle T, Preisser L, Marteau V, Meduri G, Lambert M, Nitschke R, Brown PD, Corman B. 2000 Vasopressin V1a receptor signaling in a rat choroid plexus cell line. Biochem Biophys Res Commun. ;275(2):322-7. PMID: 10964665.

Hyperlink to ECACC Cell Line Data Sheet: 00031626