RI-T
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Description
Description: Rat hepatic stellate cell(HSC) line immortalized with SV40 T antigen.
Also Known As:
Species: rat
Tissue: liver
Growth Properties:
Morphology: spindle-shape
Growth Medium: William’s E medium with 10^-6M dexamethasone, 10^-7M insulin, 25ng/ml EGF, and 10% fetal bovine serum. or RPMI-1640 medium with 10% fetal bovine serum.
Subculturing Procedure: Cells were prepared after treatment with 0.05% trypsin and 0.53mM EDTA-4Na for 3-5 min. Subculture every 4-6 days and medium change every 2-3 days. Lower concentration of cells on subculture may create colonies.
Release Conditions:
Depositor: Tokiwa,T.
Originator: Tokiwa,T. and Yata, T.
References: Follow hyperlink below to JCRB webpage for references
Hyperlink to Supplier’s Cell Line Web Page: JCRB1088
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