RT4-D6P2T
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Description
Description: Derived from the ethylnitrosourea-induced tumour line D6 of the rat peripheral nervous system. Cells will de-differentiate after 10 passages. Read references prior to use.
Also Known As:
Species: Rat
Tissue: nerve
Growth Properties: Adherent
Morphology: Bipolar
Growth Medium: DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr S Pfeiffer/Dr R Bansal, Dept. of Microbiology, Farmington
Originator: Yes
References: Proc Natl Acad Sci, USA 1978;75:6305; J Neurochem 1987;49:1902; Development 1989;106:389
Hyperlink to ECACC Cell Line Data Sheet: 93011415
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