S1814.PB5
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Description
Description: Human embryo fibroblast cell line.
Also Known As:
Species: Human
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should also grow in DMEM or RPMI 1640. This cell line also grow well in HAT.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 3-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Cytogenetics 1972;11:424 (for chromosome preparations on embryonic fibroblasts only)
Hyperlink to ECACC Cell Line Data Sheet: 85011402
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