Saos-2
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Description
Description: Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.
Also Known As:
Species: Human
Tissue: Bone
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Fogh J/ Trempe G (1975) In; Human Tumour Cells In Vitro (ed) Fogh J, Plenum Press, NY pp115-159; J Nat Cancer Inst 1977;58:209; J Nat Cancer Inst 1977;59:221
Hyperlink to ECACC Cell Line Data Sheet: 89050205
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