Description: This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated (stellate / bipolar) morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at ECACC. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.

Also Known As:

Species: Rat

Tissue: Nerve sheath

Growth Properties: Adherent

Morphology: Flattened or crescent shaped

Growth Medium: Ham’s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split semi-confluent cultures 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C Saturation density is 10,000 cells/cm².

Release Conditions: No

Depositor:

Originator:

References: Haynes LW, Rushton JA, Perrins MF, Dyer JK, Jones R, Howell R. 1994. Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo. J Neurosci Methods. 52(2):119-27. PMID: 7967716.

Hyperlink to ECACC Cell Line Data Sheet: 93031204