Description: This cell line is a derivative of the Sf9 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf9 (Spodoptera frugiperda) insect cell line. The cell line Sf9 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf9 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

Also Known As:

Species: Insect

Tissue: Ovary

Growth Properties: Semi-adherent

Morphology: Spherical

Growth Medium: TiterHigh Sf Insect Medium (Sigma I5408) no supplements.

Subculturing Procedure: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium. Incubate flask at 27°C; 5% CO2.Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.Once normal cell growth has been established maintain cultures between 3×100,000 to 1×1,0,000 cells/ml; 27°C, 5% CO2. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.5%DMSO/92.5% 1:1 conditioned media:fresh media.

Release Conditions: No

Depositor: Dr. E. Penn, European Collection of Cell Cultures, Health Protection Agency, Porton Down, Salisbury, SP4 0JG, UK

Originator: Yes

References: Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P. 1977 The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 13(4):213-7 PMID: 68913.

Hyperlink to ECACC Cell Line Data Sheet: 05011001