Sf9
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Description
Description: Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems.
Also Known As:
Species: Insect
Tissue: ovary
Growth Properties: Adherent
Morphology: Spherical
Growth Medium: TC100 + 2mM Glutamine. (The medium can be supplemented with 10% Foetal Bovine Serum (FBS) if required).
Subculturing Procedure: Maintain cultures between 3×100,000 to 1×1,0,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 9% DMSO/91% FBS. Cultures may take up to 10 days to recover normal growth characteristics following cryopreservation and resuscitation.
Release Conditions: No
Depositor:
Originator: No
References: Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P. 1977 The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 13(4):213-7 PMID: 68913.
Hyperlink to ECACC Cell Line Data Sheet: 89070101
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