Description: SK-GT-4 was established from a primary tumour in 1989 from a 89 year-old Caucasian male who presented with dysphagia secondary to a well-differentiated adenocarcinoma arising in the Barrett epithelium of the distal oesophagus. The tumour invaded into but not through the muscle layer (T2) and involved 3 of 14 lymph nodes (N1). SK-GT-4 was found to be tumorigenic in athymic nu/nu mice.

Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer.

Also Known As:

Species: Human

Tissue: Distal Oesophagus

Growth Properties: Adherent

Morphology: Epithelial growing in islands

Growth Medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cell population doubling time 39 hours.

Release Conditions: No

Depositor: Dr W.N.M. Dinjens, Department of Pathology, Josephine Nefkens Institute, Erasmus MC, University Medical Centre, Rotterdam PO Box 2040, 3000CA Rotterdam. Originator: Prof N Altorki Weill Cornell Medical Centre, 525 East 69th Street Box 110 New York NY 10065.

Originator:

References: Altorki N, Schwartz GK, Blundell M, Davis BM, Kelsen DP, Albino AP. 1993 Characterization of cell lines established from human gastric-esophageal adenocarcinomas. Biologic phenotype and invasion potential. Cancer.72 (3):649-57 PMID: 8334620. Rodriguez E, Rao PH, Ladanyi M, Altorki N, Albino AP, Kelsen DP, Jhanwar SC, Chaganti RS.1990 11p13-15 is a specific region of chromosomal rearrangement in gastric and esophageal adenocarcinomas. Cancer Res. 50(19):6410-6 PMID: 2400998. Schwartz GK, Jiang J, Kelsen D, Albino AP. 1993 Protein kinase C: a novel target for inhibiting gastric cancer cell invasion. J Natl Cancer Inst. 85 (5):402-7 PMID: 8433394.

Whole-genome sequencing of nine esophageal adenocarcinoma cell lines

Hyperlink to ECACC Cell Line Data Sheet: 11012007