SNL 76/7
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Description
Description: SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.
Also Known As:
Species: Mouse
Tissue: Embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split confluent cultures 1:5-1:15 i.e. seeding at 2-4×10,000 cells/cm² using trypsin/EDTA;5% CO2; 37°C.
Release Conditions: No
Depositor: Profesor Allan Bradley, The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK
Originator: Yes
References: Thomas and Capecchi (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51(3): 503-12.
Hyperlink to ECACC Cell Line Data Sheet: 07032801
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