SW 1417
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Description
Description: Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.
Also Known As:
Species: Human
Tissue: colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin/EDTA; No CO2; 37°C. Centrifugation and resuspension are recommended to disperse clumps.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1976;36:4562
Hyperlink to ECACC Cell Line Data Sheet: 90102543
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