TA3 Hauschka
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Description
Description: In form of tumorigenic ascites shows diminished expression of histocompatibility antigens and readily overcomes histoincompatibility barriers. Immunoresistant TA3/Ha sub-line arose spontaneously without subjection to any known immunological selection pressure.
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Species: Mouse
Tissue: breast
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow partly in suspension, therefore spin down supernatant at time of subculture to collect the cells.
Release Conditions: No
Depositor:
Originator: No
References: Trans NY Acad Sci 1953;16:64; J Exp Med 1972;135:839
Hyperlink to ECACC Cell Line Data Sheet: 85061102
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