Description: Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.

Please note: Due to difficulties that can be experienced during the initial start-up of this cell line, this cell line is provided as a LIVE CULTURE.

Also Known As:

Species: Human

Tissue: blood

Growth Properties: Suspension

Morphology: Monocyte

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure:

1. Starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube.

2. Take a sample of the cell suspension, e.g. 100ul, to count cells.

3. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes.

4. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 20% serum.

5. Incubate flask at 37°C; 5 – 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days.

6. Once the culture is established the serum concentration can be reduced to 10%.

7. To keep the cells in exponential growth, maintain cultures between 3-8×100, 000 cells/ml.

8. Requires 5% DMSO and 95% foetal bovine serum (FBS) as cryoprotectant.

Release Conditions: No

Depositor:

Originator: No

References: Int J Cancer 1980;26:171; Cancer Res 1982;42:1530; J Immunol 1983;131:1882

Hyperlink to ECACC Cell Line Data Sheet: 88081201