Description: UKRV-Mel-2 (ESTDAB-109) was established from the pleural effusion of a 36 year-old female patient with metastatic melanoma (skin stage IV). The UKRV-Mel-2 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB (http://www.ebi.ac.uk/ipd/estdab) contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. This information is appended below. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. Robinson J, Waller MJ, Fail SC, Marsh SG. 2006 The IMGT/HLA and IPD databases. Hum Mutat 27(12):11929.PMID: 16944494. Pawelec G, Marsh SG. 2006 ESTDAB: a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer. Immunol Immunother. 55(6):623-7.PMID: 16421722. Robinson J, Roberts CH, Dodi IA, Madrigal JA, Pawelec G, Wedel L, Marsh SG.2009 The European searchable tumour line database. Cancer. Immunol Immunother. 58(9):1501-6. PMID: 19172270.

Also Known As:

Species: Human

Tissue: Pleural effusion

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) seed new cultures at 1.5-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density 10 x 10,000 cells/cm².

Release Conditions: No

Depositor: Prof D Schadendorf, Department of Dermatology, University of Duisburg-Essen, Essen, Germany.

Originator: Yes

References: Artuc M, Nürnberg W, Czarnetzki BM, Schadendorf D 1995 Characterization of gene regulatory elements for selective gene expression in human melanoma cells. Biochem Biophys Res Commun. 213(2):699-705. PMID: 7646526

Hyperlink to ECACC Cell Line Data Sheet: 13012436