ULK2 KO MEF (SIM)
Mouse embryonic fibroblast", autophagy, Unc51-like kinase," spontaneuosly immortalised
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Description
Description: Mouse embryonic fibroblasts (MEFs) derived from ULK1(+/-)ULK2(+/-) knockout mice. MEFs that were isolated and cultured were immortalised by standard serial passaging protocol. This cell line can be used to study ULK2-dependent processes in autophagy.
Also Known As:
Species: Mouse mixed genetic background (Blk6/129 Agouti)
Tissue: Embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 10% FCS + 2mM Glutamine
Subculturing Procedure: Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 10e4 per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37°C.
Release Conditions: Yes – commercial organisations are required to complete the 'Cancer Research Technology Deposit Release Form for Commercial Organisations'
Depositor: Cancer Research Technology Limited
Originator: Yes
References: McAlpine F, Williamson LE, Tooze SA, Chan EY. Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. Autophagy 2013 Mar 9(3):361-73 PMID: 23291478
Hyperlink to ECACC Cell Line Data Sheet: 14050810
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