Description: Vero cell line (ECACC catalogue no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent i.e. adherent and may be suitable for the replication of viral particles.

Also Known As:

Species: Monkey

Tissue: Kidney

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4mM Glutamine. When freezing cells down use Hektor G medium + 4mM Glutamine + 10% DMSO.

Subculturing Procedure: In order to prevent syncitia formation, do not allow cells to become confluent. Split semi-confluent cultures 1:3 i.e. seeding at 4×10,000 cells/cm² using an animal component free dissociation reagent; incubate at 37°C. Please note: Once cells have detached from the flask it is necessary to neutralise the dissociation reagent using a compatible inhibitor . Centrifuge the cells; 100-150 x g for 3-5 minutes; count and re-suspend at the recommended seeding density in fresh medium.

Release Conditions: Yes – CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the European Union

Depositor: Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies Inc., Via al Chioso 14, 6929 Gravesano, Switzerland, Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland, and Ing. Claudio Strebel of CePower Inc., Einsiedlerstr. 29, 8820 Waedenswil, Switzerland. Supported by the Swiss Foundation FFVFF .

Originator: Yes

References: None Specified By Depositor

Hyperlink to ECACC Cell Line Data Sheet: 03092503