W6/1
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Description
Description: The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A. Application: human erythrocyte marker.
Also Known As:
Species: Mouse
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Seed at 5 x 10,000 cells/ml. Subculture when cells reach 5 x 100,000 – 1 x 1,0,000 cells/ml (usually after 3-4 days), 5% CO2; 37°C. Start in 20% FBS when recovering from frozen.
Release Conditions: Yes
Depositor:
Originator: No
References: Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A. 1978 Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 14(1):9-20. PMID: 667938.
Hyperlink to ECACC Cell Line Data Sheet: 13082201
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