Description: Although originally described as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29 (Chen TR, Drabkowski D, Hay RJ, Macy M, Peterson W Jr. 1987 WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet Cytogenet. 27(1):125-34. PMID: 3472642). HT29 was isolated from a primary colon adenocarcinoma in a 44 year old Caucasian female.  The cells produce carcinoembryonic antigen and are tumorigenic in xenogeneic animals. They have been used in studies of anti-tumour agents.

Also Known As:

Species: Human

Tissue: colon

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: In Vitro 1979;15:401; Cancer Res 1974;34:105; Cancer Res 1978;38:3745, Science 1985; 230:943-945, Cytogenet. 1987; 27:125-134, Cancer Res 1993; 53:5697-5706, Proc. Natl. Acad. Sci USA 1990; 87:7555-7559, Science 1978; 199:980-983

Hyperlink to ECACC Cell Line Data Sheet: 85111501