WISH (HeLa derivative)
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Description
Description: Originally derived from human amnion tissue. Have been used in virus studies; susceptible to VSV (Indiana)”, adenovirus 3 and poliovirus. Useful in differentiating virulent and avirulent measles virus. HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:10 i.e. seeding at 1-5×10, 000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Exp Cell Res 1961;23:14; Nature 1976;259:211
Hyperlink to ECACC Cell Line Data Sheet: 88102403
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