WRL 68 (HeLa derivative)
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: The human hepatic cell line WRL-68 exhibits a morphology similar to hepatocytes and hepatic primary cultures. Cells have been shown to secrete albumin and alpha-feto protein and express liver specific enzymes such as alanine amino transferase, aspartate amino transferase, gamma-glutamyl transpeptidase and alkaline phosphatase. Previously contaminated with Mycoplasma; treated and cured at ECACC. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-5×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: US Patent No 3,935, 066; In Vitro Cell Dev Biol 1994;30A:366
Hyperlink to ECACC Cell Line Data Sheet: 89121403
Ask a Question
Have a question about this product? Our technical team is here to help.
