Description: This cell line is a cloned strain from an adrenal cortex tumour cell line of a male LAF1 mouse. This strain was isolated after alternate passage in tumours in vivo and in cultures in vitro (Proc Natl Acad Sci USA 1962;48:1184-1190). The isolated clones are reported by the original depositor to produce steroid hormones at a high rate (4ug/mg/hr) under ACTH stimulation and cells round up within minutes of ACTH treatment. The hormone produced is reported to be ketosteroid with a hydroxy group at the carbon 20 rather than the usual ketosteroid configuration. However, a laboratory independent to the depositor has since (Dec 2000) tested this steroid producing property using the same method (Cancer Res 1966;26:529) and after 1 hour of stimulation could report no rounding up or change in morphology and a very low cAMP measurement. This laboratory did find that the cells round up when exposed to forskolin (acting through adenylate cyclase) and the original depositor reported morphological changes in response to cholera toxin.

Also Known As:

Species: Mouse

Tissue: adrenal cortex

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: Ham’s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Cancer Res 1966;26:529, Proc Natl Acad Sci USA 1962;48:1184-1190, Compendium of methods for the microbiological examination of foods , 3rd ed. Washington, DC: American Public Health Association; 529-535,1966,”

Hyperlink to ECACC Cell Line Data Sheet: 85062807