YTA 3.1.2
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Description
Description: The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.
Also Known As:
Species: Rat
Growth Medium: Iscoves Modified Dulbeccos Medium (IMDM) + 2mM glutamine + 5% Foetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Saturation density 9 x 100,000/ml
Release Conditions: Yes
Depositor: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge
Originator: Yes
References: Qin S, Cobbold S, Tighe H, Benjamin R, Waldmann H. 1987 CD4 monoclonal antibody pairs for immunosuppression and tolerance induction. Eur J Immunol. 17(8):1159-65 PMID: 2441998.
Hyperlink to ECACC Cell Line Data Sheet: 89040603
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