YTH 71.3
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Description
Description: Rat hybridoma producing antibody against human CD66 (human carcinoembryonic antigen CEA). Cambridge Enterprise Reference number AB64:www.enterprise.cam.ac.uk.
Also Known As:
Species: Rat
Growth Medium: Iscoves Modified Dulbeccos Medium (IMDM) + 2mM glutamine + 5% Foetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Saturation density 9 x 100,000/ml
Release Conditions: Yes
Depositor: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge
Originator: Yes
References: Watt SM, Sala-Newby G, Hoang T, Gilmore DJ, Grunert F, Nagel G, Murdoch SJ, Tchilian E, Lennox ES, Waldmann H. 1991 CD66 identifies a neutrophil-specific epitope within the hematopoietic system that is expressed by members of the carcinoembryonic antigen family of adhesion molecules. Blood. 78(1):63-74 PMID: 1712645.
Hyperlink to ECACC Cell Line Data Sheet: 11072205
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