YTH 906.9HL
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Description
Description: Rat hybridoma producing antibody against human CD25. The designation HL indicates that it has bselected for loss of the original Y3 myeloma derived (non-specific) light chain. Cambridge Enterprise Reference number AB61:www.enterprise.cam.ac.uk.
Also Known As:
Species: Rat
Growth Medium: Iscoves Modified Dulbeccos Medium (IMDM) + 2mM glutamine + 5% Foetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml. 5% CO2; 37°C. Saturation density 9 x 100,000/ml
Release Conditions: Yes
Depositor: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge
Originator: Yes
References: Tighe H, Friend PJ, Collier SJ, Decurtins M, Lim S, Cobbold SP, Thiru S, Calne RY, Waldmann H. 1987 Delayed allograft rejection in primates treated with anti-IL-2 receptor monoclonal antibody Campath-6. Transplantation. 45(1):226-8 PMID: 3122385.
Hyperlink to ECACC Cell Line Data Sheet: 11060809
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