Z41
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Description
Description: The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. The IgM antibodies bind murine IgG2a of “a” allotype and Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.
Also Known As:
Species: Mouse
Growth Medium: RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr E Rajnavölgyi, Dept. of Immunology, L.Eötvös University, Göd
Originator: Yes
References: Eur J Immunol 1990;20:2719; Mol Immunol 1990;27:1241; Immunol 1995;84:645; Intl Immunol 1995;7:125
Hyperlink to ECACC Cell Line Data Sheet: 93022520
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