Z41

CODE: 93022520
Mouse Sp2/0 x Mouse BALB/c spleen

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Description

Description: The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. The IgM antibodies bind murine IgG2a of “a” allotype and Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.

Also Known As:

Species: Mouse

Growth Medium: RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C.

Release Conditions: No

Depositor: Dr E Rajnavölgyi, Dept. of Immunology, L.Eötvös University, Göd

Originator: Yes

References: Eur J Immunol 1990;20:2719; Mol Immunol 1990;27:1241; Immunol 1995;84:645; Intl Immunol 1995;7:125

Hyperlink to ECACC Cell Line Data Sheet: 93022520

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