143B
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Description
Description: Cells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (ECACC Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+(Skovgaard et al 2010 PMID: 20544518).
Also Known As:
Species: Human
Tissue: bone
Growth Properties: Adherent
Morphology:
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 15ug/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Obtained from ATCC
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 91112502
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