149BR
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Description
Description: Initiated on 6th November 1987 from a biopsy of a 51 year old normal male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB182 (ECACC catalogue no. 98062317) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex
Originator: Yes
References: Green MH, Arlett CF, Cole J, Harcourt SA, Priestley A, Waugh AP, Stephens G, Beare DM, Brown NA, Shun-Shin GA 1991 Comparative human cellular radiosensitivity: III. Gamma-radiation survival of cultured skin fibroblasts and resting T-lymphocytes from the peripheral blood of the same individual. Int J Radiat Biol. 59(3):749-65 PMID: 1672363.
Hyperlink to ECACC Cell Line Data Sheet: 90011807
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