161BR
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Description
Description: Established from a skin of a normal 49 year old male. The cells have been used in radiation survival studies.
Also Known As:
Species: Human
Tissue: skin
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex
Originator: Yes
References: Green et al.,” (1991) Comparative human cellular radiosensitivity: III. Gamma-radiation survival of cultured skin fibroblasts and resting T-lymphocytes from the peripheral blood of the same individual. Int J Radiat Biol 59:749?65.
Hyperlink to ECACC Cell Line Data Sheet: 90011810
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