1777N Rpmet
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Description
Description: This cell line was established from a retro-peritoneal metastasis of a non seminomatous germ cell carcinoma.
Also Known As:
Species: Human
Tissue: Germ cell tumour
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.
Release Conditions: No
Depositor:
Originator: No
References: Bronson et al., 1983. In vitro differentiation of human embryonal carcinoma cells. In: Teratocarcinoma Stem Cells. Cold Spring Harbour Conferences on Cell Proliferation, Vol. 10 (LM Silver, GR Martin, S Strickland, eds.), pp 597-605
Hyperlink to ECACC Cell Line Data Sheet: 06011801
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