1BR3
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Description
Description: Derived from a skin biopsy of a normal adult male. The cells have been used in cell survival and mutation studies. The genetic identity of 1BR3 (90011801)”, 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.
Also Known As:
Species: Human
Tissue: Skin
Growth Properties: Attached
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr C Arlett, MRC Cell Mutation Unit, University of Sussex
Originator: Yes
References: Arlett et al.,” (1988) Comparative human cellular radiosensitivity: I. Int J Radiat Biol 54:911-28. (PMID: 2903889)
Hyperlink to ECACC Cell Line Data Sheet: 90011801
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