22Rv1
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Description
Description: 22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor beta-1 (TGF beta-1). This cell line is tumorigenic in nude mice.
Also Known As:
Species: Human
Tissue: Prostate
Growth Properties: Adherent aggregates
Morphology: Epithelial
Growth Medium: RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: James W. Jacobberger, Case Western Reserve University, Cleveland, Ohio, USA
Originator: Yes
References: Sramkoski et al.: A new human prostate carcinoma cell line, 22Rv1. In Vitro Cell. Dev. Biol. Anim. 35: 403-409, 1999.
Hyperlink to ECACC Cell Line Data Sheet: 05092802
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