Description: The human embryo kidney cell line 293 (ECACC catalogue no. 85120602) adapted to grow in the chemically defined protein- and peptide-free Hektor G medium (Cell Culture Technologies). Cells grow in suspension and tend to form aggregates in static culture. The cells grow as finely dispersed single cell suspension under optimal agitated culture conditions.

Also Known As:

Species: Human

Tissue: Kidney

Growth Properties: Aggregates in suspension

Morphology: Fibroblast

Growth Medium: Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4mM Glutamine + 5 ug/ml Insulin. The exogeneous insulin supplementation can be reduced or totally eliminated under optimal culture conditions. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO.

Subculturing Procedure: Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 100,000 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cell clumps form. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C.

Release Conditions: No

Depositor: Joint deposit from: Dr. Ferruccio Messi of Cell Culture Technologies LLC, Via al Chioso 12, 6929 Gravesano, Switzerland, and Rene Fischer of the Laboratory for Organic Chemistry, ETH Zurich, 8093 Zurich, Switzerland. Supported by the Swiss Foundation FFVFF (Fonds Fuer VersuchstierFreie Forschung), Hegarstr. 9, 8032 Zurich, Switzerland.

Originator: Yes

References: None Specified By Depositor

Hyperlink to ECACC Cell Line Data Sheet: 05030204