Description: Hybrid generated by fusion of 6-thioguanine-resistant mutant of mouse neuroblastoma N4TG3 (ECACC catalogue number 08062515) and 8-bromodeoxyuridine-resistant mutant of mouse L cell fibroblast line B82 (ECACC catalogue number 08062522) using inactivated Sendai virus. The fusion of N4TG3 x B82 generated two types of hybrid cells: neuronal resembling the neuroblastoma and epithelioid with clearly visible nucleus. 328/9 is of the neuronal type.

Also Known As:

Species: Mouse

Tissue: Neuronal/fibroblastic

Growth Properties: Adherent

Morphology: Neuronal/fibroblastic

Growth Medium: DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Originator: Yes

References: Hamprecht, (1977) Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture Int. Rev. Cytol. 49: 99-170.

Hyperlink to ECACC Cell Line Data Sheet: 08062533