33B
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Description
Description: 33B is a N-Ethyl-N-nitrosourea-induced neural tumour cell line derived from the 17th in vivo passage of TR33B. The cell line is positive for rat neural antigen-1 (Ran-1) and also reacts with a marker for normal Schwann cells, the monoclonal antibody 217c.
Also Known As:
Species: Rat
Tissue: nerve
Growth Properties: Adherent
Morphology: Neuronal
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr K Fields, Albert Einstein College of Medicine, Bronx, New York
Originator: Yes
References: Neuroscience 1985;15:877-885
Hyperlink to ECACC Cell Line Data Sheet: 85081901
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