3A(tPA-30-1)
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Established by SV40 ts30 transformation of human placental cells. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage.
GMO1
Also Known As:
Species: Human
Tissue: Placenta
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Alpha-MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6×10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 36°C. Change medium 2-3 times weekly.
Release Conditions: No
Depositor:
Originator: No
References: Chou JY, 1978. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75:1409-1413.
Hyperlink to ECACC Cell Line Data Sheet: 96020926
Ask a Question
Have a question about this product? Our technical team is here to help.
Related products
-
1.4E7
CODE: 10070102A human pancreatic Beta cell: PANC-1 hybrid cell line. Insulin-secreting cell line. -
-
