Description: Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 hours. We would like to manage customer expectations with regard to the potential of the current 3T3 cell line stocks to differentiate into adipocytes. If you intend to use the cells for adipocyte differentiation please note: When cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g. 2 to 5 weeks, and the proportion of the population which differentiates can be limited. If you have previously used 3T3 cells from an alternative source we cannot guarantee the differentiation performance will be the same. We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.

Also Known As:

Species: Mouse

Tissue: Adipose

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: DMEM + 10% Newborn Calf Serum (NCS) + 2mM Glutamine.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA, 5% CO2, 37°C. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use Newborn calf serum NCS + 10% DMSO, freeze down at 30-50% confluency.

Release Conditions: No

Depositor:

Originator: No

References: Green and Kehinde 1974. Cell 113-116, Green and Kehinde (1975) Cell 5 19-27, Green and Kehinde (1976) Cell 7 105-113.

Hyperlink to ECACC Cell Line Data Sheet: 00070654