3T3 Swiss Albino
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Description
Description: Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.
Also Known As:
Species: Mouse
Tissue: embryo
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5×1,000 to 1×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Virology 1965;27:179; J Cell Biol 1963;17:299; PNAS USA 1964;51:66
Hyperlink to ECACC Cell Line Data Sheet: 85022108
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