54SCC2
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Description
Description: Hybrid generated by fusion of bromodeoxyuridine-resistant mutant of rat glioma cell line C6 (C6-BU-1, ECACC catalogue number 08062563) and the 6-thioguanine-resistant mouse L cell fibroblast line A9 (ECACC catalogue number 08062526) using inactivated Sendai virus. Cells formed from such a fusion are multipolar and extend small processes.
Also Known As:
Species: Mouse x rat hybrid
Tissue: Fibroblastic/glioma
Growth Properties: Adherent
Morphology: oligopolar
Growth Medium: DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany
Originator: Yes
References: Hamprecht, (1977) Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture Int. Rev. Cytol. 49: 99-170.
Hyperlink to ECACC Cell Line Data Sheet: 08062560
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