Description: Derived from the non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.

Also Known As:

Species: Rat

Tissue: thyroid

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells need to be grown on flasks coated with matrigel or any other attachment factor

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. For cell attachment culture vessels need to be coated with collagen type IV e.g. Sigma product C-5533. Soak in a solution at 2.5ug/ml overnight at +4C. Alternatively, Matrigel may aid cell attachment. Cells tend to grow as loosely attached single cells, or in clumps.

Release Conditions: No

Depositor:

Originator: No

References: Endocrinology 1980;107:509; Proc Natl Acad Sci, USA 1980;77:3741

Hyperlink to ECACC Cell Line Data Sheet: 87042206