Description: 1.2B4 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line (ECACC catalogue number 93121055). 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.2B4 cells provide a method of producing pure insulin secreting cells when stimulated (please see attached protocols for the stimulation of insulin secretion).. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes.

Also Known As:

Species: Human

Tissue: Pancreatic islets

Growth Properties: Adherent

Morphology: Epitheloid

Growth Medium: RPMI-1640 + 2mM Glutamine + 10% FCS

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 100,000 cells/cm² can be expected.

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor:

Originator:

References: Nishimura N et. al.,” (1993) Newly established human pancreatic carcinoma cell lines and their lectin binding properties Int J Pancreatol 13: 31-41 PMID: 8454916.

Hyperlink to ECACC Cell Line Data Sheet:  10070103