AtT-20/D16v-F2
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Description
Description: This F2 subclone was developed from original AtT-20, an andrenocorticotropic hormone (ACTH) secreting cell line established from murine pituitary tumour. These cells are readily transfected using a standard calcium phosphate protocol.
Also Known As:
Species: Mouse
Tissue: pituitary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% FBS.
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5, seeding at 1 x 10,000 – 3 x 10,000 cells/cm², using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cell clumps must be broken up to prevent cells growing in suspension.
Release Conditions: No
Depositor:
Originator: No
References: Cell 1982;28:51; Cell 1983;35:531; J Cell Biol 1985;101:639
Hyperlink to ECACC Cell Line Data Sheet: 94050406
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