Description: Derived from trypsinised skin. The cells entered a ‘crisis’ period after 3 passages and subsequently stabilised. Remain stable for at least 100 passages. Susceptible to rabies virus, producing similar titres as those from Vero cells.

Also Known As:

Species: Rabbit

Tissue: embryo

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Prof F Horaud, Institut Pasteur, Paris

Originator: Yes

References: Dev Biol Standard 1985;60:133

Hyperlink to ECACC Cell Line Data Sheet: 89020802