HT29 gluc C1
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Description
Description: A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
Also Known As:
Species: Human
Tissue: colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D Louvard, Dept. of Molecular Biology, Institute Pasteur
Originator: Yes
References: J Cell Biol 1987;105:345; J Biol Chem 1991;266(7):4495
Hyperlink to ECACC Cell Line Data Sheet: 92012401
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