IEC 18
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Description
Description: Derived from normal epithelial cells of the rat ileum. Cells should undergo at least 20 population doublings. The line was established using the same techniques as for IEC6 (ECACC catalogue no. 88071401). IEC 18 cells grow in small islands and do not reach confluency.
Also Known As:
Species: Rat
Tissue: Ileum
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Release Conditions: No
Depositor:
Originator: No
References: J Cell Biol 1979;80:248: J Nat Cancer Inst 1981;67:1353
Hyperlink to ECACC Cell Line Data Sheet: 88011801
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