K1
Human thyroid carcinoma
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Description
Description: Derived from a primary papillary thyroid carcinoma cell line GLAG-66 (Antonini et al 1993 PMID: 8330267) (Ribeiro et al. 2008 PMID: 19087340 Schweppe et al. 2008 PMID: 18713817). Retains thyroid follicular cell differentiation e.g. thyroglobulin synthesis. Expresses wild-type p53 tumour suppresser gene.
Also Known As:
Species: Human
Tissue: Thyroid
Growth Properties: Adherent
Morphology:
Growth Medium: DMEM:Ham's F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine
Originator: Yes
References: Wyllie FS, Lemoine NR, Barton CM, Dawson T, Bond J, Wynford-Thomas D. 1993 Direct growth stimulation of normal human epithelial cells by mutant p53. Mol Carcinog: 7(2):83-8 PMID: 8457292
Hyperlink to ECACC Cell Line Data Sheet: 92030501
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