L-2
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Description
Description: Derived from normal rat lung tissue. The cells possess elevated levels of specific enzymes involved in the synthesis of phosphatidyl choline. Osmiophilic lamellar inclusions are observed in cell pellets from mass culture.
Also Known As:
Species: Rat
Tissue: lung
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Coon’s modified Ham’s F12 or Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Cell Biol 1973;59:160a; In Vitro 1974;10:230
Hyperlink to ECACC Cell Line Data Sheet: 90112811
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