M1WT3
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Description
Description: Derived in 1990 by transfecting Chinese Hamster ovary CHO-K1 cells with the gene encoding rat m1 muscarinic acetylcholine receptor (mAChR) in the mammalian expression vector, pSVL. These cells were then co-transfected with pMSVNeo and selected for growth in selection medium containing 500µg/ml G418. The cells are reported to express 800 fmol of receptor/mg of membrane protein.
Also Known As:
Species: Hamster
Tissue: ovary
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Ham’s F12 + 2mM Glutamine + 50-100µg/ml G418 + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-2×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Biochem Biophys Res Comm 1990;173:666
Hyperlink to ECACC Cell Line Data Sheet: 940822109
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