Description: Derived by SV40 transformation of the line BALB/3T3 clone A31. The cells exhibit a high saturation density, lack contact inhibition and form tumours in appropriate hosts.

Also Known As:

Species: Mouse

Tissue: embryo

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 10% New Born Calf Serum (NBCS) or (5% NBCS + 5% Foetal Bovine Serum (FBS)).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1×1,000 to 3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Cell Physiol 1968;12:141; Science 1968;162:1024

Hyperlink to ECACC Cell Line Data Sheet: 90030801