A-72
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Description
Description: Derived from tumour of thigh (unknown histology) of 8 year old Golden Retriever which had a previous history of mammary adenocarcinoma. Susceptible to canine corona-“, canine Ad type I and II, canine herpes, canine parainfluenza and canine parvo- viruses.
Also Known As:
Species: Dog
Tissue: thigh
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5×10, 000 cells/cm² using 0.25% trypsin; 0.02% EDTA; 37°C. CO2 is detrimental; free gas exchange with atmospheric air necessary.
Release Conditions: No
Depositor:
Originator: No
References: Am J Vet Res 1980;41:55
Hyperlink to ECACC Cell Line Data Sheet: 89050908
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