A-72

CODE: 89050908
Canine Golden Retriever tumour

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Description

Description: Derived from tumour of thigh (unknown histology) of 8 year old Golden Retriever which had a previous history of mammary adenocarcinoma. Susceptible to canine corona-“, canine Ad type I and II, canine herpes, canine parainfluenza and canine parvo- viruses.

Also Known As:

Species: Dog

Tissue: thigh

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5×10, 000 cells/cm² using 0.25% trypsin; 0.02% EDTA; 37°C. CO2 is detrimental; free gas exchange with atmospheric air necessary.

Release Conditions: No

Depositor:

Originator: No

References: Am J Vet Res 1980;41:55

Hyperlink to ECACC Cell Line Data Sheet: 89050908

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